Studiing the effect of exerciise on the expressiion of iinduciiblle niitriic oxiide synthase and heat shock proteiin 70 iin the braiins of miice wiith iinduced Parkiinson`s diisease

Abstract

Parkinson disease is a common neurodegenerative disease. Deficiency of dopamine is thought to be responsible for development of Parkinson Disease. Exercise training has been associated with improvements in patients with Parkinson Disease. The objective of the present study is to explore the effect of exercise training on the expression of HSP70 and iNOS in brains of mice with induced Parkinson Disease. Forty albino mice were selected and assigned into four groups: Sedentary control (SC, N=10), exercised control (EC, N=10), Parkinson Disease (PD, N=10) and exercised Parkinson Disease (EPD, N=10). MPTP protocol was used to induce Parkinson Disease by injections of 10 doses of MPTP (25 mg/kg) and probenecid (250 mg/kg) over 5 weeks. After the protocols treadmill exercise training had been finished, samples from the brain tissues were assessed by immunohistochemistry to examine the expression of HSP70 in the four groups of animals. The results of the present study showed that the expression of HSP70 was reduced in the brain of mice with Parkinson Disease significantly (P < 0.05) compared with control groups. The results also showed that exercise training increased the expression of HSP70 in EC significantly (P < 0.05) compared with control group, and insignificantly (P>0.05) in EPD compared with PD. Although the increased expression of HSP70 in exercised Parkinson Disease was not significant, it has a potential role in improved the status of mice with Parkinson Disease and it may have a potential therapeutic role. The data of the present study also showed significant expression of iNOS in brainsof rats with induced Parkinson Disease compared with control group (P 0.000) compared with control group, and this expression was significantly decreased in exercised group (P 0.000).