Evaluation de l’activité anti-inflammatoire de l’extrait hydro-méthanolique des graines de Lepidium sativum « Etude expérimentale chez les souris »

Abstract

Lepidium sativum of the Brassicaceae family, is used to treat inflammatory conditions such as arthritis. The purpose of this study is to evaluate the in vivo anti-inflammatory activity of the Lepidium sativum seed hydromethanolic extract on the model of acute inflammation of the mouse foot edema induced by Carragenan. And to confirm anti-inflammatory activity a histophatological study was carried out at the end of the experiment. The acute toxicity assessed on mice showed that the hydromethanolic extract of Lepidium sativum seeds produced no toxic effects at 300 mg/kg body weight. NMRI mice were selected and experiments were conducted in three groups, the therapeutic dose group (150 and 300mg/kg), the standard group and the control group. In the 1% Carragenan-induced leg edema, the test extract produced moderate anti-inflammatory activity, the percentage inhibition of edema volume with the 300mg/kg extract tested and Diclofenac (50mg/kg) at the 5th hour was 86.85% and 83.49%, respectively. As a result, the anti-inflammatory effect of the Lepidium sativum seed hydromethanolic extract appears to be more effective than that of standard (Diclofenac). However, the effect did not show statistically significant activity due to the variation in standard group data. A histophatological study carried out on the skin tissue of the mouse paw confirms the anti-inflammatory effect of the hydromethanolic extract of the seeds of Lepidium sativum.