La Spermoculture Chez Le Cheval

Abstract

Sperm culture is an examination to help diagnose certain infections (whether or not it induces a male fertility problem). It generally accompanies or follows a spermogram or spermocytogram, which are first-line examinations, which can also be supplemented by examinations aimed at assessing the fertilizing functions of the spermatozoon (Cf. oocyte penetration capacities), or those involved in mobility. necessary for crossing the female genital tract. Sperm culture guides the andrologist's choice towards antibiotic therapy. A negative sperm culture does not guarantee the absence of infection, especially in the presence of leukocytosis. Freezing sperm causes a significant decrease in metabolism or even a complete stop. The aim of the study was to evaluate the zootechnical performance of dairy cows and to determine the strengths and weaknesses of the dairy cattle herd management with regard to reproductive management, particularly the synchronization of heat, reproductive performance and productivity of females. The study was based on the use of the following protocols (PRID Delta, use of prostaglandin F2 Alpha, GPG protocol). Cooling leads to a change in membrane composition, causing membrane alteration or even rupture.This change in membrane composition can lead to a "false" acrosomal reaction and thus reduce the life span of the sperm and its ability to fertilise, this is called cryocapacitation. The centrifugation step is carried out after the semen is harvested and its purpose is to separate the seminal fluid from the sperm and to improve the quality of the refrigerated or frozen semen. However, centrifugation causes loss of sperm when the force used is not adapted. New centrifugation te chniques are increasingly being used such as "cushion" centrifugation, which reduces sperm damage, or colloid centrifugation and filtration to select high quality semen . Conditioning is the fractionation of semen to make it more easily storable, identifiable and usable. There are two types of conditioning in different species: pellets and straws, with straws being the most commonly used nowadays. The volume of the straws as well as the concentration of sperm in the straws have an influence on the quality of the semen. In general, 0.25 milliliter straws are used with a concentration of 200 million spermatozoa per milliliter. In recent years the genetic origin of some stallion sub-fertility problems has been studied through emerging technologies. Other studies, aimed at improving the understanding of male fertility, have addressed the molecular mechanisms regulating the development and function of spermatozoa. This work could thus be of interest to the standard, although caution should be exercised when extrapolating results from one species to another