Etude de l'activité antimicrobienne et antioxydant de l’extrait méthanoliques de l’algue rouge : Sphaerococcus coronopifolius

Abstract

Aquatic environments are home to organisms rich in compounds endowed with biological activities, presenting an enormousre source of new compounds with significant potential; among these organisms are macroalgae which are a potentially rich source of bioactive compounds such as isoprenoids and polyphenols. In this present work we evaluated the extraction rate, the rate of phenolic compounds, as well as the antimicrobial activity of methanolic extracts of the marine alga Sphaerococcus coronopifolius which is tested against 6 microorganisms including two ( 2) Gram positive bacteria (+): B. cereus, and S. aureus, and three (3) Gram negative bacteria (-) P. aeruginosa, K. pneumoniae and E. coli as well as a fungus: ayeast C. albicans. The results indicate a yield of crude extractis (3.4%). Analysis of phenolic compounds shows that the methanolic extractobtained from the red alga S. coronopifolius has a high content of total flavonoids (10172μg EQ / mg extract) relative to total phenols (7066μg EA / mg extract). The results of the antimicrobial activity of the crude extract of S. coronopifolius showed the following inhibition diameters: E. coli obtained 30 mm, S. aureus recorded an inhibition diameter of the order of 25 mm, B. cereus in turn marked a diameter of the order of 22.7 mm, C. albicans marked 17 mm, K. pneumoniae marked 15 mm and P. aeruginosa marked 0 inhibition diameter. The results of this work have enabled us to affirm that the antioxidant activity of the extract of red algae S. coronopifolius essentially returns to the phenolic compounds, and that it has a significant inhibitory effect against certain pathogens.