Résumé:
Aquatic environments contain organisms rich in compounds endowed with biological
activities presenting an immense resource of new compounds with significant capacities;
among these organisms are algae which are a possibly rich source of bioactive compounds.
In this present work we evaluated the extraction rate, phytochemical screening and the rate of
phenolic compounds, as well as the in vitro anti-inflammatory activity of methanolic extract
of the seaweed Sphaerococcus coronopifolius which is tested with two different methods:
evaluation of its protective properties against thermal denaturation of BSA albumin and
against heat-induced hemolysis of red blood cells.
The results indicate a yield of crude extract is 1.6%. Phytochemical screening revealed the
presence of some bioactive substances: alkaloids, glycosides and saponins. The quantification
of total polyphenols and flavonoids gave a high value in total polyphenols 582.78 Ec μg EAG
/ mg of extract compared to that of total phenols 70.544 μg EQ / mg extract.
However, the results of the anti-denaturing activity of the proteins of this species are
IC50=8.51±1.84μg /ml. On the other hand, the results obtained during the anti-hemolytic test
by heat gave IC50=71.39±0.21μg/ml.
Sphaerococcus coronopifolius species which is rich in bioactive substances has antiinflammatory
activity
