in vitro and in vivo control of Fusarium spp. of durum wheat (Triticum durum) using fungal endophytes isolated from algae

Abstract

We tested the antagonistic capacities of 15 isolates of endophytic fungi collected from marine algae from the Stidia region of Mostaganem against 3 isolates of Fusarium sp. pathogen of durum wheat. These isolates were isolated from infected samples of wheat collected from El-Hadjaj El-Menea. In this experiment we did a test in vitro to identify the antagonistic endophytes between the 15 isolates of our collection against 3 Fusarium spp. The tests are carried out using an antibiosis test. Endophytes that have shown an antagonistic effect in vitro are selected for an in vivo test by testing their behaviour on wheat plants inoculated with one of the 3 parasites. Test in vivo includes biopriming on seeds and immersing method for leaves and plants using sporal suspension 10⁶ spores/ml of the Fusarium isolates and antagonistic isolate. The results obtained in vivo negative results of using endophytic antagonist on plant material including decrease in germination rate and increase of disease severity on leaves and plants