Abstract:
Objective: To investigate the effect of unheated and heat-treated of Sahara honey.
Methods: A total of 24 Swiss albino mice weighing 25–35 g were divided into four groups (n
= 6). Anti-inflammatory effect was assessed at 1, 2, 3, 4, 5 and 6 h after subplantar injection of carrageenan (0.5 mL of a 1% solution in normal saline). In addition, total phenolic content was determined by modified Folin-Ciocalteu method.
Results: The total phenolic content capacity of the Sahara honey before and after heat
treatment was between 72 and 97.9 mg of gallic acid equivalents/100 g of honey respectively.
Administration of unheated honey (oral administration) reduced significantly (P < 0.05).
The carrageenan induced mice paw edema model at 1, 3 and 6 h for 21.85%, 5.43% and
80.43%, respectively. Administration of heat-treated honey showed insignificant inhibition
of carrageenan and induced paw edema at 1 h (31.16%), 3 h (0.25%) and 6 h (34.19%). The
50 mg/kg diclofenac exhibited percent reduction in paw volume 16.12%, 8.90% and 15.32%
after 1 h, 3 h and 6 h, respectively, when compared with control animals. No toxicity was
identified.
Conclusions: Our results suggest that unheated Sahara honey has anti-inflammatory effects
by reducing the mice paw edema size while heat-treated Sahara honey decreases the antiinflammatory activity.
