Abstract:
Soil pollution by oil is an environmental and health problem. It is therefore necessary
to decontaminate polluted environments. Bioremediation proved to be a very interesting
alternative. In this study, we identified two originally isolated bacterial isolates from soil
contaminated by oil (P6, B1). Identification is based on morphological studies (macroscopic
and microscopic examination) and biochemical (deferent biochemical tests to identify the
orientation of our isolates). In addition to these tests based on the morphology of the bacteria
we used galleries API 20 E and API 20 NE to determine the genus and species of our isolates.
P6 strain was related to the genus Pseudomonas. The inability of this strain to degrade
mannitol and positive oxidase test led us to suppose that this is Pseudomonas luteola. B1
strain was related to the genus Bacillus. The inability of this strain to degrade mannitol and
negative oxidase test allowed us to assume that it is Bacillus thuringiensis. Both isolates were
exposed to increasing doses of ultraviolet radiation in order to establish the survival curve of
each strain.
