Résumé:
The aim of the current study was to evaluate the total phenolic, flavonoid content and to
investigate the antioxidant capacities of the fruit extracts of Arbutus unedo L. that grows in Tiaret
area (Western Algeria). First we have extracted the fruit by some non-polar solvent (chloroform,
ethyl acetate, 1-butanol).
Total phenolic content and total flavonoid content were evaluated according to the Folin-
Ciocalteu procedure, and a colorimetric method, respectively. Extracts content was determined by
using a high-performance liquid chromatography (HPLC)-UV method. The total phenolic contents
of A.unedo L. varied between 12.75±0.06 to 34.17±1.36 mg gallic acid equivalent/g of dry weight of
extract. The total flavonoid varied from 2.18±0.10 to 6.54±1.14 mg catechin equivalent/g.
The antioxidant potential of all extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free radical scavenging activity, the IC50 of acetate ethyl was the best by 0,009 mg/ml may
due to the phenolic compound, in the second was the chloroform extract by IC50=0,015mg/ml, in
the third was butanol extract by IC50= 0,022 mg/ml and in the last was water extract by IC50=
0,048mg/ml. the antioxidant activity of all extracts was better than ascorbic acid. The extract
obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were
identified; they are catechin, apiginin, silybin, fisetine and naringin.
