Résumé:
The use of microorganisms for remediation and restoration of hydrocarbons contaminated soils is an effective
and economic solution. The current study aims to find out efficient telluric filamentous fungi to degrade petroleum hydrocarbons pollutants. Six fungal strains were isolated from used engine (UE) oil contaminated soil.
Fungi were screened for their ability to degrade crude oil, diesel and UE oil using 2.6-dichlorophenol indophenol
(DCPIP). Two isolates were selected, identified and registered at NCBI as Aspergillus ustus HM3.aaa and
Purpureocillium lilacinum HM4.aaa. Fungi were tested for their tolerance to different concentration of petroleum
oils using radial growth diameter assay. Hydrocarbons removal percentage was evaluated gravimetrically. The
degradation kinetic of crude oil was studied at a time interval of 10 days. A.ustus was the most tolerant fungi
to high concentration of petroleum oils in solid medium. Quantitative analysis showed that crude oil was the
most degraded oil by both isolate; P. lilacinium and A. ustus removed 44.55% and 30.43% of crude oil, respectively.
The two fungi were able to degrade, respectively, 27.66 and 21.27% of diesel and 14.39 and 16.00% of UE oil. As
compared to the controls, these fungi accumulated high biomass in liquid medium with all petroleum oils. Likewise, crude oil removal rate constant (K) and half-lives (t1/2) were 0.02 day−1
, 34.66 day and 0.015 day−1
,
46.21 day for P. lilacinium and A. ustus, respectively. The selected fungi appear interesting for petroleum oils biodegradation and their application for soil bioremediation require scale-up studies
