Study of antioxidant properties of five Algerian date (phoenix dactylifera L) cultivars by cyclic voltammetric technique

Abstract

Phoenix dactylifera L.(date palm) is well known for its innumerable health benefits and nutritional values. Therefore, the present work aimed to determine the total phenolic content in five date palm fruit (DPF) varieties, Degla Baidha (DB), Deglet Nour (DN), Ghars (Gh), Tamjhourt (Tam), and Tafezauine (Taf), from the Ouargla region (Algeria), extracted with aqueous methanol (80%) and to evaluate in vitro their antioxidative properties by cyclic voltammetry technique (CV) in aprotic media. Consequently, the total polyphenol contents (TPC) of these extracts will be measured using Folin Ciocalteu spectrophotometric method. Total phenolic content ranged from 5.9 to 23.05 mg gallic acid equivalents (GAE/100g). Thereafter, the voltammetric response of the electrochemically gener-ated superoxide anion radical in DMF was monitored in the absence and presence of the DPF extracts. The decrease in the current was interpreted in terms of antiradical activity of the added extract. Effective scavenging concentration (IC50) on superoxide anion radical ranged from 33.17-85.23 mg/l, the IC50 values decreased in the order of TafGhDBTamDN. Finally, the use of DPF extracts for protection against oxidative stress has been recommended.