Evaluation de l’effet inhibiteur del’inflammation de l’extrait hydro-ethanolique de Cichorium spinosum L. chez la souris

Abstract

Cichorium spinosum L. is a wild plant that is attracting interest in therapeutic alternatives. The objective of this study is to evaluate in vivo the anti-inflammatory activity of the hydroethanol extract of C. spinosum. The experimental protocol was established according to the edema of the mouse paw induced by a phlogistic agent. The animals were divided into five groups, all of which underwent an intra-articular injection of the 1% carrageenan solution in the paw. One hour prior to induction of inflammation, the CSD1, CSD2 and CSD3 groups received the hydroethanol extract of C. spinosum at 50, 150 and 250mg/kg respectively by gastric gavage. The standard group (STD) was treated with a reference drug (Diclofenac at 50mg/kg). As for the inflammation control group (Ti), it received distilled water. The measures of paw edema determined by the percentages of increase (%AUG) and inhibition (%INH) were the criteria for evaluating the inflammation inhibitory effect of C. spinosum extract. These parameters were supported by histological study of the paw tissue. The results of %AUG recorded in CSD2 and CSD3 groups a significant decrease from the 3rd hour compared to the Ti group. Nevertheless, the administration of C. spinosum extract at 250mg/kg resulted in a significantly higher edema inhibitory activity at the 5th and 6th hour compared to the STD group. The histological examination of the paw confirmed the results of the macroscopic analysis of the edema, presenting a regular aspect and without distinct anomalies caused by carrageenan in the group treated with C. spinosum extract at 250mg/kg. The results obtained in our study, allow us to grant the anti-inflammatory effect to the extract of C. spinosum, which would probably be conferred by its phytochemical compounds. These results would be promising for the elaboration of possible therapeutic formulations.