Effet anti-inflammatoire de l'extrait hydroéthanolique de Cichorium spinosum L. sur les oedèmes de la patte induits par la carragénine (étude expérimentale chez la souris)

Abstract

Many plants are known to be used in traditional medicine for the treatment of several pathologies including diseases with an inflammatory component. The aim of this study is to evaluate the in vivo anti-inflammatory activity of the hydro ethanolic extract of Cichorium spinosum L. cultivated in Europe on the model of acute inflammation of the mouse paw edema induced by Carrageenan 1%. A histological study was performed at the end of the experiment to confirm the results of the increase and inhibition of the mouse paw volume. Acute toxicity evaluated on mice showed that the hydroethanol extract of Cichorium spinosum L. did not induce any toxic effect at the dose of 1000 mg/Kg body weight. NMRI mice were selected and experiments were carried out in three groups treated with therapeutic doses of (50, 150 and 250mg/kg) compared to the standard group which receives a synthetic anti-inflammatory treatment (diclofenac 50mg/kg) and the positive control group (carrageenan 1%) The results obtained show that the extract produced a moderate anti-inflammatory activity, the percentage of inhibition of the volume of edema with the extract tested at the dose of 250 mg/kg and Diclofenac (50mg/kg) at the 5th hour (70%), this percentage of inhibition is high compared to Diclofenac which is about 56%. From these results it can be said that the antiinflammatory effect of the hydroethanolic extract of Cichorium spinosum L. seems to be effective than that of the standard (Diclofenac). However, the effect did not show statistically significant activity due to the variation in the data of the standard group. A histopathological study carried out on the skin tissue of the mice's paw confirms the antiinflammatory effect of the hydro-ethanolic extract of Cichorium spinosum L. and this following the almost total disappearance of edema and inflammatory infiltrate in the group of mice treated with the dose of 250 mg/kg at the end of experimentation and which encourages in the future its application in the therapeutic field for the treatment of different inflammatory diseases.