inventaire des plantes à caractère médical rencontrées dans les forêts de Mostaganem et l’’activité antibactérienne des deux plantes de la famille des Cistacées

Abstract

The nosocomial infection becomes a real problem that has been worrying doctors for a very long time; it can only be completely eliminated if the draconian conditions of absolute sterilization are met, which is far from being the case. The experiementation is carried out on the inhibiting effect of Cistus monspeliensis and Helianthemum sp are rough extracts on the growth of Staphulococcus aureus, Pseudomonas and E. Coli. The Cistus monspeliensis and Helianthemum sp are rough extracts are diluted at some 10; 10-1; 10-2; 10-3; 10-4 rates. The following measures and controls (Diameters of inhibition, test of growth, test of CMI and CBM) were tested on Staphulococcus aureus, Pseudomonas and E. Coli. The largest diameter of zone of inhibition is notes to 100% of oil of cists, in the order of 28 mm and helianthus of 24 mm on average, the phenol extract of cists , E. Coli (12mm) ; Pseudomonas (18mm); Staphulococcus aureus (22mm), and extract of helianthus, E. Coli (17mm) ; Pseudomonas (20mm) ; Staphulococcus aureus (20mm), in the same way, the best results of the rates of inhibitions are obtained at the solutions of extractions prepare to 80 and 100%; respectively and diminution of growth at (30. 105 à 0 UFC/ml). Lastly, the inhibiting minimal concentration and bactericidal minimal concentration are obtained at to solutions of extraction prepared to 80 and 100%; our extracts seem to exert a bactericidal effect on the germs studied. Our results confirm that the plants Cistus monspeliensis and Helianthemum sp of the family Cistaceae have doses of polyphenols (Ciste = 11,603, Helianthème = 11,091%) and have a very important antibacterial activity vis-à-vis the strains responsible for nosocomial infections and an antioxidant activity Helianthemum sp (Rf = 0.13, 0.75, 0.88, 0.94), Cistus monspeliensis (Rf = 0.1, 0.66, 0.75, 0.88, 0.96) [methanolic extracts]. Helianthemum sp (Rf = 0.09, 0.5, 0.72), Cistus monspeliensis (Rf = 0.09, 0.68, 0.89) [chloroform extracts]