Contribution à l’étude de l’effet antidiabétique du miel saharien et du pollen Etude in vivo chez le rat « Wistar »

Abstract

The traditional medicine offers alternative remedies such as: medicinal plants and natural products which honey is one of the most important. They possess hypoglycemic properties to control or treat diabetes disease and its complications. Therefore, they are enriched bya lot of antioxidants that ensure prevention or delay of different pathologies. The study’s objective is to evaluate the antidiabetic activity of two natural products which are honey and pollenin induced diabetes model. After 21 days of prolative treatment with euphorbia honey(10%), multifloral pollen extract (1%), and the mixture – honey and pollen- in male rats by oral pathway, the streptozotocine (STZ) in proportion of 60 mg/kg body weight is induced by intraperitonial pathway (IP) with one dose. The toxicity test ofthe selected doses represents no effect in all experimental animals. Before induction with STZ, the glucose levelis stable in all tested rats. Otherwise, after diabetes induction, the increasing level of glucose shows ahigher significance in diabetic rats (D) comparatively with control subjects (C). However, the treated rats with honey (DH) and with the mixture –honey and pollen- (DHP) have shown an hypoglycemic effect appearing in a significant low glucose level comparing with diabetic rats (D). The histological examination shows damages in the pancreatic tissuein diabetic rats (D) with comparison with controls (C). Whereas, the histological aspect of pancreas in diabetics treated with honey (DM) and treated with the mixture –honey and pollen- (DMP) seem to be very approach to control rats’ pancreatic aspect. The histological study of hepatic tissue in diabetic rats (D) and diabetics treated with pollen(DP) demonstrate a moderate hepatitis with lymphocytic infiltration more noticed in diabetics (D). The histology’s results reflect the results of biochemical parameters essentially blood glucose concentrationwhich confirmed the antidiabetic activity of the studied honey. This study shows that the euphorbia honey represents a better antidiabetic effect under the conditions of our experimentation.