Contribution à l’étude de l’effet de céleri (Apium graveolens L) sur les bactéries responsables des infections urinaires.

Abstract

The active principles of plants with significant antibacterial activity and can substitute successfully with antibiotics that show their inefficiencies against resistant microorganisms. This led us to conduct the study of the antibacterial activity of essential oil, vegetable oil extracted from Apium graveolen L which is obtained by different extraction methods, and the comparative study with antibiotics: ampicillin and gentamicin prescribed to treat urinary tract infections, this effect was studied on two bacterial strains Staphylococcus aureus and Escherichia coli. According to the results we note that essential oil yield is about 1.95% and in line with international standards. The results of physicochemical analysis of the essential oil conformed to standards, the antimicrobial activity has yielded interesting results: the inhibition of Escherichia coli by of 11 mm diameter area corresponding to an inhibition rate of 32.35 %, Staphylococcus aureus by 8 mm diameter region corresponding to an inhibition rate of 47.05%. The results of the extract in a yield of 10.4% on Escherichia coli give a 19 mm diameter zone corresponding to an inhibition rate of 55.88% for Staphylococcus give a diameter of 17 mm corresponding to a inhibition rate of 94.11%. The results of the plant celery oil with a yield of 1.13% Escherichia coli gives a zone of diameter 15 mm to an inhibition rate of 44.11% for Staphylococcus aureus diameter 10 mm corresponding to an inhibition rate of 58.82%. In addition, we conducted a phytochemical study on medicinal plant tested for the detection of certain active ingredients that could be responsible for the observed inhibitory effect on the bacterial strains