PHENOLICS CONTENTS AND IN-VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF THE AERIAL PARTS OF THREE ALGERIAN ATRIPLEX HALIMUS L. ECOTYPES

Abstract

Atriplex halimus L. “Guettaf”, halophyte plants, is distributed in different geographical zones in Algeria. In this study, antiradical activities, polyphenols, flavonoïds, flavonols and carotenoïds contents of aerial parts (Leaves and Stems) from three Algerian sites (Mazagran, Biskra and Sig) were determined. Results revealed higher amounts of secondary metabolites and higher antioxidant capacities in leaves extracts unlike stems. Significant variation was found between ecotypes; Mazagran leaves and stems extracts are richer in TPC (279.33±1.17 and 68.11±7.76 mg GAE/g LE) and TFC (98.19±1.51and 64.35±0.41 mgRE/g LE, respectively). Great concentrations of flavonols and carotenoids were exhibited in Mazagran and Sig leaves extracts, respectively (34.87 ±1.32 mgRE/g LE and 73±00 µgCE/g LE). Like TAC, DDPH, ABTS and BCB tests demonstrated the higher antiradical capacity of Biskra stems extracts (IC50 = 497.46±37.53 TEµg/mL - 56.75±5.36 TEµ mol/mL and 17.38±4.17 BEµg/mL, respectively). Biskra Atriplex halimus can be used as source of natural antioxidant, in different fields such as animals’ food and medicinal applications.