Traitement d'Escherichia coli et Pseudomonas aeruginosa par UV : mise en point d'une courbe de mortalité.

Abstract

This thesis focuses on the impact of ultraviolet (UV) rays on two common bacteria in the environment and in the medical field, Escherichia coli and Pseudomonas aeruginosa. UV rays have the ability to damage the DNA of living organisms, which can inactivate bacteria and decrease their viability. In this study, two isolates of Escherichia coli and Pseudomonas aeruginosa were exposed to ultraviolet radiation for different periods of time in order to establish a mortality curve for the two strains. Our results showed that Escherichia coli and Pseudomonas aeruginosa, both species, are sensitive to ultraviolet rays. UV treatment significantly reduced the number of viable colonies of both bacteria, suggesting effective bactericidal action. Ultraviolet radiation is one of the physical factors that cause mutations. This study was based on naked eye examinations. The results showed a difference in bacterial colony growth with increasing UV exposure time.