La Résistance des Pseudomonas au cuivre

Abstract

Pseudomonas species are among the bacteria that secrete siderophores to meet their iron needs. Charged siderophores are internalized across the outer membrane after attachment to specific receptors. This iron acquisition system can be exploited for bioacumulated heavy metals including copper. The objective of this work is to isolate and characterize bacteria from the rhizosphere of a wild grass plant. pseudomonas-like isolates were tested for resistance to copper, these bacteria were tested by Benaied and Meddah ( ) at different doses ( ; 5; 8; 6; 6; 7; 8 and 8 mM) of copper sulphates (CuSO7, 8H5O), showing resistance to copper especially for isolates K and . The Determination of the minimum inhibitory concentration (CMI) of copper sulphate vis-à-vis the most efficient isolates indicates that the two fluorescent Pseudomonas bacteria (k and ) are inhibited by the concentration of 9 mM. The evidence of the effect of copper sulphate on the growth of the two fluorescent Pseudomonas species isolated in the two different mediums (King B and BN) shows that copper has a growth-slowing effect.