Phytochimie et Activité Anti-hémolytique des Extraits de l’Alaterne « Rhamnus alaternus » L

Abstract

The aim of the present work is to identify phytochemical compounds using a colorimetric method based on specific tests, and to estimate in vitro the anti-hemolytic activity of extracts from the aerial part of a medicinal plant: Rhamnus alaternus L. against hemolysis induced by hypotonia, H2O2 and heat. Phytochemical screening identified a diversity of secondary metabolites: terpenoids, quinones, saponins and reducing compounds were found to be highly positive, followed by flavonoids and tannins, which were predominant in aqueous infusion extracts. Sterols were not detected. The results of the anti-hemolytic activity against hypotonic hemolysis show that the infusion extract diluted to 25% gave good erythrocyte protection with a maximum of 97.92% (0.2% NaCl). The protection of plant extracts against H2O2 is positive for all dilutions of extracts, with a maximum at the 25% dilution for most tests. Rates of anti-hemolytic activity in the presence of Rhamnus extracts against heat indicate that whatever the dilutions for all extracts, at temperatures of 45C° and 55c° protection is marked for all extracts and dilutions. At 35°C, anti-hemolysis activity is inhibited.