Etudein vivo et in vitro de l’efficacité de l’extrait de Mentha spicata sur les larves de Tuta absoluta sur tomate sous serre.

Abstract

The tomato leafminer T. absoluta remains the most feared pest by farmers, because the intensive use of chemicals has caused contamination of fauna and flora as well as the appearance of resistance phenomenon in insects. Medicinal and aromatic plants represent an immense reservoir of biologically active natural molecules. It is in this line that our work was carried out to evaluate the effect of polyphenol extract of Mentha spicata on T. absoluta larvae in vitro and in vivo, to meet this objective, a maceration extraction. Cold leaves of M. spicata were carried out, to test it by spraying on tomato leafminer larvae. The polyphenol extract of mint has shown remarkable efficiency compared to controls. The results obtained show the L1 instars sensitivity and the 20% concentration efficiency in vitro as well as the results obtained in vivo show the changes in the evolution of T. absoluta as a function of time and the concentrations thus used according to the distribution blocks. We recorded a 35.65% infestation rate before treatment. At the end of the experiment, the infestation rate was 2.55% on the treated blocks and 14.87% for the T- (water) and 13% for the T + (methanol). The polyphenol extract has shown remarkable efficacy against the larvae of tomato leafminer