Effet de l’huile essentielle d’une mixture de plantes médicinales sur la croissance des germes multi résistants des infections nosocomiales

Abstract

The current study is centered on the valorization of a mixture consisting of eight medicinal plants, namely Thymus vulgaris, Ammi visnaga, Mentha pulegium, Zingiber officinale, Cinnamomum verum, Syzygium aromaticum, Lavandula angustifolia, and Citrus sinensis, through the assessment of their various biological characteristics. The essential oil extracted from the mixture via hydro-distillation exhibited a yield of 2.4%. Analysis through phytochemical screening unveiled the existence of diverse classes of secondary metabolites in the upper parts of the plants like alkaloids, flavonoids, and tannins. Evaluation of antimicrobial effects using an aromatogram demonstrated a substantial inhibitory impact of the combined oils on the five standard strains tested, with inhibition zone diameters ranging from 9 mm to 34 mm. Among the strains, Staphylococcus aureus, Escherichia coli, exhibited the highest sensitivity with inhibition zone diameters of 24.13 mm, 18.99 mm, respectively, while P.aeruginosa displayed the least sensitivity with an inhibition zone diameter of 9.45 mm. Through the liquid macrodilution technique, minimum inhibitory concentration (MIC) values were determined to be in the range of 0.62 to 2.5 μL/mL with a minimum bactericidal concentration (MBC) of 5 μL/mL. Notably, our sample showcased significant anti-radical activity against the DPPH radical, with an IC50 value of 1.97 ± 0.2 mg/mL. Additionally, the EC50 acquired from the iron reducing power test (FRAP) was calculated to be 1.763±0.08 mg/mL. The oral administration of our essential oil to Wister rats at escalating doses was found to be moderately toxic, with an LD50 falling between 50 and 2000mg/kg.