Effet des [lavonoïdes et des tanins sur le remodelage de la matrice extracellulaire au cours du processus de cicatrisation cutanée

Abstract

Plants used in traditional pharmacopoeia represent a considerable resource of bioactive molecules of interest to the pharmaceutical and cosmetics industry. This study focused on the extraction of polyphenols and tannins from the bark of pomegranate (Punica granatum L.) and carob (Ceratonia siliqua L.), two Algerian medicinal plants. Two extraction methods were compared: conventional solid-liquid extraction and ultrasound-assisted extraction. Deep eutectic solvents were used as ecological alternatives. Quantitative results revealed that for carob, total polyphenols contents were 78,880± 11,002( mg EAG/ ml) (water bath) and 100,146 ±31,172 (mg EAG/ml) (ultrasound), 8lavonoïdes 1. 445± 0.262 ( mg EQ/ml) (ultrasound) and 1.010 ± 0.158 (mg EQ/ml) (water bath).hydrolysable tannins from 5.838± 1.529 ( mg EAT/ ml )(water bath) and 16.221± 0.912( mg EAT/ml)(ultrasound). For pomegranate, total polyphenols were 41.126± 11.231 (mg EAG/ml) (water bath) and 72.593± 2.456 (mg EAG/ml) (ultrasound), 8lavonoïdes 0. 845± 0.111 (mg EQ/ ml) (water bath) and 1,206± 0.179 (mg EQ/ ml) (ultrasound), and hydrolysable tannins from 5,824± 0.971 (mg EAT / ml) (water bath) and 7,485± 0.324 (mg EAT/ml) (ultrasound). Evaluation of biological activities revealed strong antioxidant and anti-in8lammatory activity of extracts obtained by ultrasound, particularly with ethanol-ED