Abstract:
Hydrocarbons are a major pollutant of soils. Biodegradation of petroleum products through soil microorganisms has emerged as a method of economic and ecological interest. The purpose of our study was to isolate and identify the bacterial species present in the soil polluted by hydrocarbons: crude oil. We identified nine (09) bacterial isolates isolated initially from petroleum-contaminated soil (C1, C2, C3, C4, C5, C6, C7, C8, C9). The identification is based on morphological studies (study macroscopic and microscopic) and biochemical (the deferent biochemical tests of orientation to identify our isolates).
The capacity of the isolated strains to degrade the crude oil was carried out in a solid medium by a spray method and the bacterial growth was followed in a liquid medium supplemented with a single source of carbon: the oil, by UV spectrometry.
