Résumé:
In this work, we test a GFCO ointment from the Algerian pharmacopoeia based on pomegranate bark, Aloe ferox dried gel, extra virgin olive oil, bee wax and dromedary fat. The objective was to evaluate and prove its anti-hemorrhoidal effectiveness enhanced by an oral intake of the strain of Lacticaseibacillus rhamnosus SL42, known for its probiotic effects. In vitro, polyphenols, flavonoids, and the antioxidant activity of the ointment were quantified. In addition, we evaluated its healing and hemostatic activities. In vivo, Wistar rats were used to create an animal model of hemorrhoids by applying COP and acetic acid and were subsequently treated with GFCO ointment for 14 days. On the day of sacrifice, we had measured various parameters evaluating treatment, including inflammatory scores, rectal histology, and changes in intestinal flora. The results revealed significant antioxidant power; with high levels of polyphenols and flavonoids. Analysis of hydrogen peroxide trapping capacity and astringent activity confirmed the efficacy of the ointment in reducing inflammation and healing. Furthermore, our results also showed that GFCO ointment combined with the SL42 probiotic strain effectively reduced (90%) hemorrhoids in rats whose intestinal content reflected a high charge of lactic bacteria. These results suggest that the combination of GFCO ointment with probiotic may offer an effective and natural treatment of hemorrhoids. These promising results pave the way for further research into the molecular mechanisms involved in this restorative effect. Clinical trials in humans are highly needed to confirm the effectiveness and safety of this ethnomedical treatment.
