Résumé:
Lactic acid bacteria have long played an important role in the food industry. Probiotics, which include bacteria from genera such as Lactobacillus, Lactococcus and Bifidobacterium, possess antimicrobial properties and are widely used in the food industry. These bacteria play a crucial role in various ecosystems and have a positive impact on human health.
Our research focused on the extraction of lactic strains from samples of raw camel and goat milk collected in the LAGHOUAT and MOSTAGANEM regions. This procedure yielded a total of 15 Gram-positive and catalase-negative strains. In our study, we focused on examining the ability of the lactic acid bacteria obtained to combat Uro-pathogens. Using various microbiological methodologies, we succeeded in isolating 15 strains classified in the following genera:
Lactobacillus helveticus, Lactobacillus delbrueckii subsp bulgaricus, Lactococcus lactis subsp cremoris, Lactococcus lactis subsp lactis, Enterococcus faecium, Streptococcus thermophulis, Bifidobacteriumn adolescentis.
Urinary tract infections (UTIs) are a particularly important health problem, occupying a major place in infectious pathology. The majority of these infections are caused by enterobacteria (80%), of which Escherichia coli is the most commonly observed bacterium. Gram-positive cocci come in 2nd place. Using the well diffusion method, an investigation was carried out into the inhibitory impact of bacteriocin-producing lactic acid bacteria on three reference strains: Staphylococcus aureus ATCC 43300, Pseudomonas aeruginosa ATCC27853, Citrobacter freundii ATCC13316, Candida albicans ATCC10231, Klebsiella pneumoniae ATCC700603 and Proteus mirabilis ATCC35659. The results of this study showed positive and remarkable results for all the lactic bacteria isolates. The main objective of this research was to characterize the antagonistic effect of lactic strains against Uropathogens in order to select strains with a high probiotic effect.