Etude phytochimique et évaluation de l’activité antioxydante d’une plante médicinale Ruta montana collectée de la région de Mostaganem

Abstract

Ruta montana is an aromatic medicinal plant still used in traditional medicine in many countries as laxative, anti-inflammatory, analgesic, antispasmodic, abortive, antiepileptic, emmenagogue and for the treatment of cutaneous pathologies. Phytochemical screening revealed the presence of flavonoids, tannins, glycosides, sterols, coumarins and proteins and the absence of anthocyanins and quinones. The results show the richness of the aerial part of the plant in polyphenols (250 mg EAG / g MS) and flavonoids (52 mg EC / g MS) and a very small amount in tannins (0.8 mg EQ / g of MS). Antioxidant capacity was determined from IC50. Its extract concentration needed to reduce 50% of the DPPH radical. The IC50 and the antioxidant activity of the extract tested are inversely proportional. According to the results recorded, the aqueous and methanolic extracts have a moderate antioxidant capacity; their IC50 respectively is 0.945 and 0.961 mg / ml compared to the standard used in our study that of ascorbic acid whose value is of the order 0.16 mg / ml.