Identification et effet des rayons ultra-violet sur deux bactéries isolées à partir d’un sol pollué par les hydrocarbures

Abstract

Soil pollution by oil is an environmental and health problem. It is therefore necessary to decontaminate polluted environments. Bioremediation proved to be a very interesting alternative. In this study, we identified two originally isolated bacterial isolates from soil contaminated by oil (P6, B1). Identification is based on morphological studies (macroscopic and microscopic examination) and biochemical (deferent biochemical tests to identify the orientation of our isolates). In addition to these tests based on the morphology of the bacteria we used galleries API 20 E and API 20 NE to determine the genus and species of our isolates. P6 strain was related to the genus Pseudomonas. The inability of this strain to degrade mannitol and positive oxidase test led us to suppose that this is Pseudomonas luteola. B1 strain was related to the genus Bacillus. The inability of this strain to degrade mannitol and negative oxidase test allowed us to assume that it is Bacillus thuringiensis. Both isolates were exposed to increasing doses of ultraviolet radiation in order to establish the survival curve of each strain.